Scientific Publications

21 April 2018

IN VITRO EVALUATION OF THE SENSITIVITY OF A HYALURONIC ACID PEG CROSS-LINKED TO BOVINE TESTES HYALURONIDASE

Open Access Macedonian Journal of Medical Sciences. 2018 Jan 25; 6(1): 20–24.

Nicola Zerbinati,  Torello Lotti, Damiano Monticelli, Virginia Martina, Giovanna Cipolla, Edoardo D’Este, Alberto Calligaro, Roberto Mocchi, Cristina Maccario, Sabrina Sommatis, Jacopo Lotti, Uwe Wollina, Georgi Tchernev, and Katlein França

Neauvia Intense is biocompatible, injectable hyaluronic acid (HA) filler PEG cross-linked for facial soft-tissue augmentation that provides volume to tissues. The aim of the present study is to evaluate the sensitivity of Neauvia Intense in hyaluronidase from bovine testes in a time-course analysis. The test is based on the colourimetric determination of the N-acetyl – D – glucosamine (NAG) released by the hyaluronidase in standardised conditions. The in vitro conditions involve the treatment of Neauvia Intense with a known concentration of the enzyme (6080U/ml). The NAG content was determined at different times to assess the kinetics of the degradation (1h, 3h, 6h, 24h, 48h, 72h, 120h, and 168h); the Ehrlich’s reagent was used for the colourimetric quantification, by the method described by Reissing and colleagues. The intensity of the violet colour developed after the chemical reaction was proportional to the NAG present in each sample. A microplate reader at 585 nm read the absorbance. The amount of NAG released by the product was proportional to the time of incubation with bovine hyaluronidase, reaching a plateau after 168 hours.

Discover More>

CLOSE
Compile the form here to register
Those credentials are not valid!
CLOSE

Newsletter

Subscribe to the newsletter to receive the scientific and training updates from Neauvia

* indicates required
CLOSE
Compile the form here to request a reset
Those credentials are not valid!