Scientific Publications

30 June 2021

In VitroEvaluation of the Sensitivity of aHyaluronic Acid PEG Cross-Linkedto Bovine Testes Hyaluronidase

Open Access Macedonian Journal of Medical Sciences. 2018 January 21.

Nicola Zerbinati, Torello Lotti, Damiano Monticelli, Virginia Martina, Giovanna Cipolla, Edoardo D’Este, Alberto Calligaro, Roberto Mocchi, Cristina Maccario, Sabrina Sommatis,Jacopo Lotti, Uwe Wollina, Georgi Tchernev, Katlein França

ABSTRACT

Neauvia Intense is biocompatible, injectable hyaluronic acid (HA) filler PEG cross-linked for facial soft-tissue augmentation that provides volume to tissues. The aim of the present study isto evaluate the sensitivity of Neauvia Intense in hyaluronidase from bovine testes in a time-course analysis. The test is based on the colourimetricdetermination of the N-acetyl–D-glucosamine (NAG) released by the hyaluronidase in standardisedconditions. The in vitro conditions involve the treatment of Neauvia Intense with a known concentration of the enzyme (6080U/ml). The NAG content was determined at different times to assess the kinetics of the degradation (1h, 3h, 6h, 24h, 48h,72h, 120h, and 168h); the Ehrlich’s reagent was usedfor the colourimetricquantification, bythe method described by Reissing and colleagues. The intensity of the violet colour developed after the chemical reaction was proportional to the NAG present in each sample. A microplate reader at 585 nm read the absorbance. The amount of NAG released by the product was proportional to the time of incubation with bovine hyaluronidase, reaching a plateau after 168 hours.

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