Scientific Publications

30 June 2021

In Vitro Evaluation of the Biological Availability of Hyaluronic Acid PolyethyleneGlycols-Cross-Linked Hydrogels to Bovine Testes Hyaluronidase

BioMed Research International, Volume 2019, Article ID 3196723. 2019 June 13.

N.Zerbinati, R.Mocchi, H.Galadari, C.Maccario, M.Maggi, R.Rauso, A.Passi, C.Esposito, and S.Sommatis


During last years, hyaluronic acid- (HA-) based dermal fllers have grown rapidly and continuously, as reported by the American Society ofAesthetic Plastic Surgery (ASAPS). In fact, HA fllers are considered the gold standard technique for soh tissue augmentation, deep skin hydration,and facial recontouring, playing a key role as an alternative to plastic surgery. HA fllers are less invasive, more biocompatible, and safer and with amore natural and immediate result if compared to plastic surgery. Hence, the safety of HA-based dermal fllers plays a crucial role, mostly in termsof biocompatibility and adjustability in case of unpleasant results and side effects such as, tyndall effect, edema, or granulomas. Hyaluronidase is anaturally occurring enzyme, present in the human body, and can degrade HA fllers avoiding more severe complications. In this article, weanalyzed the bioavailability of hyaluronidase degradation of fve fllers of Neauvia® hydrogels line (MatexLab SA, Lugano, CH), composed of purehyaluronic acid and based on PEGDE cross-linking (polyethylene glycol) technology that guarantees a higher biocompatibility and an optimalbiointegration and rheological characteristics. ae performed in vitro testing is based on the colorimetric determination of the N-acetyl-D-glucosamine (NAG) present in solution aher incubation with hyaluronidase, determined at different time points in order to assess the kinetic ofeach product degradation (1h, 3h, 6h, 24h, 48h, 72h, 120h, and 168h). ae aim of this study was to assess, in vitro, how the difference in HAcontent and PEGDE concentration of the analyzed fllers can inluence the product biocompatibility, intended as product enzymatic clearance andduration in time. ae results demonstrated that the method was reproducible and easy to perform and that all the analyzed fllers are naturallyimmediately available for hyaluronidase-mediated degradation.

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